Human Platelet Activating Factor Acetylhydrolase 2 ELISA Kit

Supplied by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) in plasma samples, with reactivity limited to human specimens. The assay is read on a colorimetric microplate reader at 450 nm using a pre-coated 96-well microplate arranged as twelve 8-well strips.

The stated performance specifications are an assay range of 0.312-20 ng/ml and a sensitivity of 0.105 ng/ml, with a total assay time of 4h 30m. Recovery and dilution linearity have been characterised for serum, EDTA plasma and heparin plasma, with average recoveries of 91% (serum), 91% (EDTA plasma) and 84% (heparin plasma) across n=5 replicates each.

Components supplied include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers, with per-item storage conditions between +4°C and -20°C.