Human Pigment Epithelium Derived Factor ELISA Kit

For the in vitro quantitative determination of human Pigment Epithelium Derived Factor (PEDF), this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is suitable for serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids. The assay uses a pre-coated microplate platform in which an antibody specific for PEDF captures the analyte from standards and samples; a Biotinylated Anti-PEDF Antibody and HRP-Avidin conjugate are then applied, and TMB substrate produces a colour change that is read at 450 nm on a colorimetric microplate reader.

The quantitative product range is 1.87–120 ng/ml, with a reported sensitivity of 0.76 ng/ml, and the total assay time is 4h 30m. Reported average recovery values are 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma (n=5 per matrix), with linearity ranges provided for each sample type at dilutions from 1:2 to 1:16. Kit components are stored at +4°C or −20°C as instructed and should not be used past the expiration date.