Human Perilipin 3 ELISA Kit

Antibodies.com supplies this Human Perilipin 3/TIP47 ELISA Kit as a sandwich enzyme immunoassay (sELISA) designed for the in vitro quantitative determination of human Perilipin 3/TIP47 in tissue homogenates or other biological fluids. The assay uses a 96-well microplate pre-coated with an antibody specific for Perilipin 3/TIP47. Detection is performed using a biotinylated anti-Perilipin 3/TIP47 antibody followed by an HRP-avidin conjugate and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader.

The kit reports a sensitivity of 0.059 ng/ml and an assay range of 0.156–10 ng/ml. Recovery data supplied with the product indicate average values of 91% in serum, 90% in EDTA plasma and 84% in heparin plasma across serial dilutions of 1:2, 1:4, 1:8 and 1:16 (n=5). Total assay time is stated as 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed and should not be used past the stated expiration date.