Human Peptidoglycan Recognition Protein 1 ELISA Kit

Designed for the in vitro quantitative determination of human Peptidoglycan Recognition Protein (PGRP-S) in serum, plasma, cell lysates and other biological fluids, this sandwich ELISA from Antibodies.com uses a pre-coated 96-well microplate with colorimetric detection read at 450 nm. The assay is run on a standard colorimetric microplate reader with an overall assay time of 4 hours 30 minutes.

Reported performance figures include an assay range of 0.312-20 ng/ml and a sensitivity of <0.115 ng/ml. Manufacturer-supplied spike-and-recovery data (n=5) show average recoveries of 91% in serum (range 80%-102%), 91% in EDTA plasma (range 81%-101%) and 84% in heparin plasma (range 80%-89%), with dilution linearity ranges reported across the same sample types at 1:2, 1:4, 1:8 and 1:16 dilutions.

Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer concentrate, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers. Components are stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.