Human Peptidase Inhibitor 16 ELISA Kit

Designed for the in vitro quantitative determination of human peptidase inhibitor 16, this sandwich ELISA kit from Antibodies.com is validated for serum, plasma, tissue homogenates and other biological fluids. The assay employs a pre-coated microplate, a biotinylated anti-PI-16 detection antibody, and HRP-avidin with TMB substrate, producing a colorimetric signal read at 450 nm.

The standard curve covers an assay range of 0.781–50 ng/ml with a sensitivity of 0.34 ng/ml, and the complete protocol runs in approximately 4 hours 30 minutes. Average recovery is 91% for serum, 96% for EDTA plasma, and 95% for heparin plasma. Kit components are stored at +4°C or −20°C as instructed, and the reagents must not be used past the stated expiration date.