Human Pepsinogen C ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com for the in vitro quantitative determination of human Pepsinogen II/PGC in serum, plasma, tissue homogenates or other biological fluids. The assay is reactive with human samples and is read using a colorimetric microplate reader.

Manufacturer-stated performance figures include a sensitivity of 12.3 pg/ml and an assay range of 31.2-2000 pg/ml. Reported sample recovery values are: serum (n=5) 80%–102% averaging 91%, EDTA plasma (n=5) 81%–100% averaging 90%, and heparin plasma (n=5) 80%–89% averaging 84%.

The assay is performed on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a total incubation time of 4h 30m. Detection is colorimetric, using an HRP-Avidin conjugate and TMB substrate; the reaction is stopped with acidic stop solution and the optical density read at 450 nm. The principle relies on a biotinylated anti-Pepsinogen II/PGC detection antibody binding captured analyte, with colour intensity proportional to Pepsinogen II/PGC concentration. Manufacturer-listed components include a pre-coated 96-well microplate, lyophilized standard, Detection Solution A, Detection Solution B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers. The manufacturer advises storing kit components at +4°C or -20°C as instructed and not using the kit past its expiration date.