Human PDGFC ELISA Kit

From Antibodies.com comes a sandwich ELISA kit for the quantitative measurement of human PDGFC in plasma samples. The assay is performed on a pre-coated microplate platform with colorimetric detection, intended for use with a standard colorimetric microplate reader. The kit reports a sensitivity of 43 pg/ml and an assay range of 125-8000 pg/ml, with a total assay time of 4 hours and 30 minutes.

PDGFC, also referred to as Fallotein, PDGF-C, Platelet-derived growth factor C, SCDGF, Spinal cord-derived growth factor, or VEGF-E, is captured using the sandwich immunoassay principle. Standards and samples are incubated in pre-coated wells, followed by detection with a biotinylated anti-PDGFC antibody and an HRP-avidin conjugate. The enzymatic reaction produces a colour change measured at 450 nm absorbance.

Validation data includes recovery assessments at dilutions of 1:2, 1:4, 1:8, and 1:16 for serum (74-107%), EDTA plasma (79-108%), and heparin plasma (82-105%) sample types. Average recovery rates across these matrices are reported at 84-91%. Kit components should be stored as instructed, and the kit should not be used past its expiration date.