Human Paraoxonase 2 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com intended for the in vitro quantitative determination of human Paraoxonase 2 (PON2) in tissue homogenates, cell lysates, or other biological fluids. The assay employs the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate (12 x 8 well strips), using a biotinylated anti-PON2 detection antibody, an HRP-avidin conjugate, and a TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader. Reported sensitivity is less than 0.34 ng/ml, with an assay range of 0.781–50 ng/ml and a total assay time of 4 hours 30 minutes. The kit has been validated with serum, EDTA plasma, and heparin plasma samples, with reported recovery ranges of 80–102% (average 91%) for serum, 81–100% (average 90%) for EDTA plasma, and 80–89% (average 84%) for heparin plasma. Kit components should be stored at +4°C or –20°C as instructed and not used past the stated expiration date.