Human Pancreas Specific Transcription Factor 1a ELISA Kit

Distributed by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is designed for the in vitro quantitative determination of human PTF1A (Pancreas Specific Transcription Factor 1a) in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate configured as 12 x 8 well strips, with detection via a biotinylated anti-PTF1A antibody and HRP-avidin conjugate visualised through TMB substrate. Reactivity is specified for human samples.

Quantitative measurement is performed by reading the optical density absorbance at 450 nm on a colorimetric microplate reader and referring to the standard curve. The stated product range is 0.156–10 ng/ml with a sensitivity of <0.075 ng/ml, and the total assay time is 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed and must not be used past the expiration date.

Validation data from the manufacturer report sample recovery at n=5: serum averages 91% (range 83–100%), EDTA plasma averages 96% (range 90–103%), and heparin plasma averages 95% (range 89–102%). Dilution linearity is provided across 1:2, 1:4, 1:8 and 1:16 dilutions for serum, EDTA plasma and heparin plasma (n=5).