Human Palate/Lung And Nasal Epithelium Associated Protein ELISA Kit

Sandwich ELISA kit from Antibodies.com for the in vitro quantitative determination of human Plunc (Palate/Lung and Nasal Epithelium Associated Protein) in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and a biotinylated anti-Plunc antibody / HRP-avidin / TMB workflow, with absorbance read at 450 nm on a colorimetric microplate reader. Total assay time is 4h 30m. The reported assay range is 0.156–10 ng/ml with a sensitivity of <0.055 ng/ml. Dilution recovery across 1:2, 1:4, 1:8 and 1:16 dilutions (n=5) spans 74–107% for serum, 79–108% for EDTA plasma and 82–105% for heparin plasma; corresponding spike-and-recovery averages are 91% (serum), 90% (EDTA plasma) and 84% (heparin plasma). Kit components include the pre-coated microplate, lyophilized standard, detection solution A, detection solution B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, to be stored at +4°C or -20°C as instructed.