Human Osteocrin ELISA Kit

Manufactured by Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative measurement of human Osteocrin in serum, plasma, tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique on a pre-coated microplate (12 x 8 well strips), with colourimetric detection read at 450 nm using a microplate reader. The product range spans 0.156–10 ng/ml, with a reported sensitivity of <0.058 ng/ml. Total assay time is approximately 4 hours 30 minutes.

Recovery has been characterised across sample matrices: serum (n=5) ranged 80–102% with an average of 91%, EDTA plasma (n=5) ranged 81–100% with an average of 90%, and heparin plasma (n=5) ranged 80–89% with an average of 84%. Dilution linearity was assessed at 1:2, 1:4, 1:8 and 1:16 dilutions. Kit components include a pre-coated 96-well microplate, lyophilised standard (2 vials), Detection Solutions A and B (120 µl each), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml), and plate sealers (5 adhesive strips). Components are stored at +4°C or −20°C as instructed, and the kit should not be used past its expiration date.