Human Nucleolin ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Nucleolin in tissue homogenates, cell lysates, and other biological fluids. The assay employs the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate (12 x 8 well strips), with captured Nucleolin detected using a biotinylated anti-Nucleolin antibody, HRP-avidin conjugate, and TMB substrate, read at 450 nm on a colorimetric microplate reader.

The manufacturer reports an assay range of 0.156–10 ng/ml and a sensitivity of <0.057 ng/ml, with a total run time of 4 hours 30 minutes. Sample recovery data covers serum (80–102%, average 91%, n=5), EDTA plasma (81–100%, average 90%, n=5), and heparin plasma (80–89%, average 84%, n=5). Dilution linearity at 1:2, 1:4, 1:8, and 1:16 is reported for each matrix. Kit components are stored at +4°C or -20°C as instructed, and the product should not be used past its expiration date.