Human Nuclear Factor Related To Kappa B Binding Protein ELISA Kit

Antibodies.com provides a sandwich enzyme-linked immunosorbent assay (sELISA) configured for the in vitro quantitative determination of human NFRKB in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, onto which an immobilised anti-NFRKB antibody captures NFRKB from standards and test samples. Detection is achieved with a biotinylated anti-NFRKB antibody followed by an HRP-Avidin conjugate, with the enzymatic reaction visualised using TMB substrate and read as absorbance at 450 nm.

The assay reports a quantitative range of 0.312–20 ng/ml and a sensitivity of 0.127 ng/ml, with a total run time of 4 hours 30 minutes. Linearity is supported across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16, with recovery for serum ranging from 80% to 110% and average recovery values of 93% (serum), 94% (EDTA plasma), and 94% (heparin plasma). Supplied components include the pre-coated plate, lyophilised standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers, held at +4°C or –20°C as indicated.