Human Notch2 N-Terminal Like Protein ELISA Kit

Manufactured for Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human NT2NL (Notch2 N-Terminal Like Protein) in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) and a colorimetric readout at 450 nm in a microplate reader, with a total assay time of 4h 30m. A biotinylated anti-NT2NL antibody is used with an HRP-avidin conjugate and TMB substrate to generate a yellow-coloured product whose intensity is proportional to NT2NL concentration, calculated by reference to a standard curve.

Per the manufacturer's documentation, the kit has an assay range of 0.312–20 ng/ml and a sensitivity of <0.117 ng/ml. Average recovery values are reported as 91% for serum, 91% for EDTA plasma, and 84% for heparin plasma, with dilution linearity data across 1:2 to 1:16 dilutions. Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solution A (120 μl), detection solution B (120 μl), 30X wash buffer (20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml), and adhesive plate sealers, with storage at +4°C or -20°C as instructed.