Human Nischarin ELISA Kit

From Antibodies.com, this human NISCH ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) for the in vitro quantitative determination of human Nischarin (NISCH) in tissue homogenates and other biological fluids. The assay is run on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with absorbance read at 450 nm using a colorimetric microplate reader.

According to the supplied specifications, the kit has a reported sensitivity of 0.067 ng/ml and a working range of 0.156–10 ng/ml. An antibody specific for NISCH is pre-coated onto the plate; standards and samples are added, followed by a biotinylated anti-NISCH antibody and an HRP-avidin conjugate. TMB substrate is then added, producing a blue-coloured product that turns yellow upon addition of the acidic stop solution, and the NISCH concentration is calculated from the standard curve. Total assay time is 4h 30m. Sample types addressed in the manufacturer's documentation include serum, EDTA plasma, heparin plasma, cell culture supernatant, cell lysates and tissue homogenates, and kit components are stored at +4°C or -20°C as instructed.

The product is for research use only and is not intended for diagnostic or therapeutic applications.