Human Neurotensin ELISA Kit

From Antibodies.com, the Human Neurotensin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of human Neurotensin in serum, plasma or other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated anti-Neurotensin detection antibody, an HRP-avidin conjugate, and a TMB substrate, with absorbance read at 450 nm. The reported assay range is 4.68-300 pg/ml, sensitivity is <1.35 pg/ml, and typical run time is 4 hours 30 minutes. Validation data show recovery averages of 91% in serum (n=5, range 80-102%), 90% in EDTA plasma (n=5, range 81-99%), and 84% in heparin plasma (n=5, range 80-89%), with dilution linearity evaluated at 1:2, 1:4, 1:8, and 1:16. Supplied components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage at +4°C or -20°C as indicated.