Human Neuroplastin ELISA Kit

From Antibodies.com, this Human Neuroplastin (NPTN) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) intended for the in vitro quantitative measurement of human NPTN in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate format (12 x 8 well strips) combined with a biotinylated anti-NPTN detection antibody and an HRP-avidin conjugate, with the colourmetric reaction read at 450 nm on a standard microplate reader.

Quoted performance characteristics include an assay range of 0.156-10 ng/ml, a sensitivity of <0.052 ng/ml, and a total assay time of 4h 30m. Kit components are stored at +4°C or -20°C as instructed and the reagents should not be used past the stated expiration date. The product falls within the Antibodies.com Assays and Kits category.

Representative validation data reported by the manufacturer show average recoveries of 91% in serum (n=5, range 80-102%), 90% in EDTA plasma (n=5, range 81-100%) and 84% in heparin plasma (n=5, range 80-89%). Dilution linearity across 1:2 to 1:16 dilutions in the same matrices gave recovery values broadly spanning 74-108%.