Human Neuronal Pentraxin Receptor ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human Neuronal Pentraxin Receptor (NPTXR) in tissue homogenates or other biological fluids. The kit employs the sandwich enzyme immunoassay technique on a pre-coated microplate, using a biotinylated anti-NPTXR detection antibody and an HRP-avidin conjugate, with TMB substrate producing a measurable colour change read at 450nm in a microplate reader.

The quoted assay range is 0.156-10 ng/ml with a sensitivity of 0.051 ng/ml, and the standard protocol takes around 4h 30m. Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or -20°C as instructed.

Manufacturer data report recovery of 80%-102% for serum (average 91%), 81%-100% for EDTA plasma (average 90%) and 80%-89% for heparin plasma (average 84%), and the kit is suitable for use with tissue homogenates alongside other sample types.