Human Neural Cell Adhesion Molecule ELISA Kit

From Antibodies.com, this ELISA kit is designed for the quantitative measurement of human Neural Cell Adhesion Molecule (NCAM1). It employs a sandwich enzyme immunoassay format and is validated for use with serum, plasma, tissue homogenates and other biological fluids.

The kit reports a sensitivity of <0.51 ng/ml and an assay range of 1.56-100 ng/ml, with a total assay time of 4 hours 30 minutes. Detection is colourimetric, with the optical density measured at 450 nm in a standard microplate reader. Standards and samples are added to a pre-coated 96-well microplate configured as 12 x 8 well strips, and NCAM1 is detected using a biotinylated anti-NCAM1 antibody followed by an HRP-avidin conjugate, with the enzymatic reaction visualised using TMB substrate.

Kit components should be stored at +4°C or -20°C as indicated on the individual component labels, and the kit should not be used past its expiration date.