Human Nei Endonuclease VIII Like Protein 1 ELISA Kit

Antibodies.com supplies this Sandwich ELISA kit for the quantitative measurement of human NEIL1 (Nei Endonuclease VIII Like Protein 1) in tissue homogenates and other biological fluids. The assay uses a pre-coated 96-well microplate format with a Biotinylated Anti-NEIL1 detection antibody and HRP-Avidin conjugate, producing a colourimetric signal that is read at 450 nm on a standard microplate reader.

According to the manufacturer's specifications, the kit has a sensitivity of 0.062 ng/ml and an assay range of 0.156-10 ng/ml. The complete protocol takes 4 h 30 min, and kit components are stored at +4°C or -20°C depending on the reagent.

Validated recovery values are reported as 80%-102% (average 91%) for serum, 81%-100% (average 90%) for EDTA plasma, and 80%-89% (average 84%) for heparin plasma across five samples each. Dilution linearity is also demonstrated across 1:2, 1:4, 1:8 and 1:16 dilutions for all three sample types.