Human N-Acetyltransferase 8 Like Protein ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit intended for the in vitro quantitative determination of human NAT8L in tissue homogenates or other biological fluids. The kit uses a pre-coated 96-well microplate (12 x 8 well strips) and follows the sandwich enzyme immunoassay technique. An antibody specific for NAT8L is pre-coated onto the plate; standards and samples are added and the NAT8L in each is bound by the immobilised antibody. Wells are then incubated with a Biotinylated Anti-NAT8L Antibody, followed by HRP-Avidin conjugate. TMB substrate is used to visualise the HRP reaction, producing a blue colour that turns yellow on addition of acidic stop solution. Absorbance is read at 450nm in a colorimetric microplate reader, and the concentration of NAT8L is calculated by reference to a standard curve.

The stated assay range is 0.156-10 ng/ml with a sensitivity of <0.064 ng/ml, and the total assay time is 4h 30m. Reported recovery data (n=5) shows averages of 93% for serum (range 84%-102%), 95% for EDTA plasma (range 91%-100%), and 95% for heparin plasma (range 92%-98%). Linearity is reported across sample dilutions of 1:2, 1:4, 1:8, and 1:16 for each matrix. Components supplied include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and plate sealers. Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.