Human Myogenic Factor 5 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com, designed for the in vitro quantitative determination of human Myogenic Factor 5 (Myf5) in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate arranged as 12 x 8 well strips, a biotinylated anti-Myf5 detection antibody, an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader.

The stated assay range is 0.156-10 ng/ml with a sensitivity of <0.066 ng/ml, and a total assay time of 4h 30m. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Manufacturer-reported recovery values include serum (n=5) at 80%-102% with an average of 91%, EDTA plasma (n=5) at 81%-100% averaging 90%, and heparin plasma (n=5) at 80%-89% averaging 84%. Dilution linearity data are reported for serum, EDTA plasma and heparin plasma across 1:2 to 1:16 sample dilutions.