Human Myelin Proteolipid Protein Like Protein ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative measurement of human Myelin Proteolipid Protein Like Protein in tissue homogenates or other biological fluids. An antibody specific for the target is pre-coated onto a 96-well microplate arranged as 12 x 8 well strips. Standards and samples are added to the wells; bound analyte is then detected using a Biotinylated Anti-Myelin Proteolipid Protein Like Protein Antibody, followed by an HRP-Avidin conjugate and TMB substrate. After addition of an acidic stop solution, the colour change is read at 450 nm on a colorimetric microplate reader and the analyte concentration is determined from the standard curve.

The stated assay range is 0.312-20 ng/ml and sensitivity is <0.112 ng/ml. Total assay time is 4h 30m on a pre-coated microplate colorimetric platform. Kit components include the pre-coated microplate, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, stored at +4°C or -20°C as instructed.

Validation data are reported for human serum, EDTA plasma and heparin plasma, with recovery ranges of 80-102% (average 91%) in serum, 81-100% (average 90%) in EDTA plasma and 80-89% (average 84%) in heparin plasma. Dilution linearity is reported across 1:2, 1:4, 1:8 and 1:16 dilutions for each sample type.