Human MYC Associated Factor X ELISA Kit

Antibodies.com provides a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of human MYC Associated Factor X (MAX) in tissue homogenates or other biological fluids. The assay uses a sandwich immunoassay format: an antibody specific for MAX is pre-coated onto the plate, samples and standards are added to capture MAX in the wells, and detection proceeds via a Biotinylated Anti-MAX Antibody followed by HRP-Avidin conjugate. TMB substrate produces a blue colour that turns yellow on addition of stop solution; absorbance is read at 450 nm in a Colorimetric Microplate Reader, with the yellow intensity proportional to MAX concentration.

The platform is a pre-coated microplate configured as 12 x 8 well strips, and the full protocol takes 4h 30m. Reported performance includes a sensitivity of 0.059 ng/ml and an assay range of 0.156-10 ng/ml. Spiking recovery across serum, EDTA plasma and heparin plasma (n=5 each) ranges from 80% to 102%, with averages of 91%, 90% and 84% respectively. Dilution linearity data are provided at 1:2, 1:4, 1:8 and 1:16 dilutions. Kit components are stored at +4°C or -20°C as instructed, and the kit must not be used past its expiration date.