Human Multimerin 1 ELISA Kit

A sandwich ELISA from Antibodies.com for the quantitative determination of the target analyte in human samples. The product listing identifies the target as Multimerin 1, while the manufacturer's accompanying protocol describes detection of ECM. The assay is suitable for serum, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

The procedure uses a pre-coated microplate with a biotinylated detection antibody, an HRP-avidin conjugate and a TMB substrate; absorbance is read at 450 nm. The manufacturer-supplied manual reports an assay range of 31.2-2000 pg/ml, a sensitivity of 9.6 pg/ml, and a total assay time of 4h 30m. Average undiluted recoveries are reported as 91% for serum, 90% for EDTA plasma and 84% for heparin plasma. Detection is colourimetric, performed on a standard microplate reader.