Human Mucin 5 Subtype AC ELISA Kit

From Antibodies.com, this Human Mucin 5AC ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of human Mucin 5AC in tissue homogenates, cell lysates, cell culture supernates, and other biological fluids. The assay uses a pre-coated microplate format and is read on a colorimetric microplate reader at 450 nm, with the intensity of the yellow product proportional to the amount of Mucin 5AC captured in each well and quantified against a standard curve.

Key performance specifications include an assay range of 78.1–5000 pg/ml and a sensitivity of <28 pg/ml, with a total assay time of approximately 4 hours 30 minutes. The kit is validated for human samples, with reported recovery in serum (average 91%, range 80–102%, n=5), EDTA plasma (average 91%, range 81–101%, n=5), and heparin plasma (average 84%, range 80–89%, n=5), alongside dilution linearity data across serial dilutions from 1:2 to 1:16.

Supplied components include a pre-coated microplate, lyophilized standard, biotinylated detection antibody, HRP-avidin conjugate reagents, wash and sample dilution buffers, TMB substrate, stop solution, and adhesive plate sealers, with storage at +4°C or -20°C as instructed and components not to be used past their stated expiration date.