Human Mothers Against Decapentaplegic Homolog 2 ELISA Kit

Manufactured by Antibodies.com, this kit is a sandwich enzyme-linked immunosorbent assay (sELISA) for the in vitro quantitative determination of human Smad2 (Mothers Against Decapentaplegic Homolog 2) in tissue homogenates, cell lysates, or other biological fluids. It is reactive with human samples and employs a pre-coated microplate arranged as 12 x 8 well strips, read on a colorimetric microplate reader at 450 nm. The reported assay range is 0.156–10 ng/ml with a sensitivity of 0.066 ng/ml, and the total assay time is 4 hours 30 minutes.

The assay uses an immobilized anti-Smad2 capture antibody, a biotinylated anti-Smad2 detection antibody, an HRP-avidin conjugate, and a TMB substrate, with the reaction stopped by an acidic stop solution. The density of the resulting colour is proportional to the Smad2 captured in each well, and concentration is calculated by reading the absorbance against a standard curve. Reported mean recovery values across serum, EDTA plasma, and heparin plasma samples are 91%, 92%, and 93% respectively (n=5), with dilution linearity demonstrated at 1:2, 1:4, 1:8, and 1:16.

Kit components include a pre-coated 96-well microplate, lyophilised standard, Detection Solutions A and B, Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.