Human Monocyte Chemotactic Protein 3 ELISA Kit

This Antibodies.com sandwich ELISA kit enables in vitro quantitative determination of human MCP3 (Monocyte Chemotactic Protein 3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and is read on a colorimetric microplate reader at 450 nm.

The reported detection range is 15.6-1000 pg/ml with a sensitivity of 5.9 pg/ml. Total assay time is 4h 30m. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.

The sandwich enzyme immunoassay principle involves a pre-coated anti-MCP3 capture antibody, a biotinylated anti-MCP3 detection antibody, and an HRP-avidin conjugate with TMB substrate. The reaction produces a yellow product whose absorbance is proportional to MCP3 concentration.