Human Monocyte Chemotactic Protein 2 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com enables the in vitro quantitative determination of human Monocyte Chemotactic Protein 2 (MCP2) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay uses a pre-coated microplate with an antibody specific for MCP2, employing a biotinylated detection antibody and HRP-Avidin conjugate. TMB substrate produces a colorimetric reaction measured at 450 nm in a microplate reader.

With a sensitivity of 8.6 pg/ml and a standard range of 31.2-2000 pg/ml, the assay is completed in 4 hours and 30 minutes. Validation studies report recovery averages of 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%), and 84% for heparin plasma (range 80-89%). Linearity was confirmed across dilutions of 1:2, 1:4, 1:8, and 1:16 for all three sample types. Kit components are stored at +4°C or -20°C as instructed.