Human MMP2 ELISA Kit

A sandwich ELISA kit from Antibodies.com for the in vitro quantitative determination of human MMP2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. The kit is supplied in a QuickRun format with ready-to-use reagents on a pre-coated microplate platform, with a total assay time of approximately 2 hours (120 minutes). Detection is colorimetric, with absorbance read at 450 nm. The assay is reported as specific for MMP2, with no cross-reactivity with other analogs or variants.

Performance figures differ between manufacturer sources: one listing states a sensitivity of < 10 pg/ml with an assay range of 625-40,000 pg/ml, while another states a sensitivity of 0.191 ng/mL with an assay range of 0.5-32 ng/mL. Validated sample dilutions include healthy serum at 1:10-1:25 and MG-63 cell culture supernatants at 1:4-1:8. Sample recovery ranges in serum, EDTA plasma, and heparin plasma are reported in the source documentation.

The kit is shipped at +4°C; component storage is at +4°C or -20°C as instructed in the Protocol Manual. Users are advised to avoid freeze/thaw cycles and not to use the kit past its expiration date.