Human Mitogen Activated Protein Kinase Kinase 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Mitogen Activated Protein Kinase Kinase 1 (MEK1) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microtiter plate; an antibody specific for MEK1 captures the target, and detection is achieved with a biotinylated anti-MEK1 antibody, HRP-avidin, and TMB substrate, with absorbance read at 450nm.

The product range is 0.156-10 ng/ml, with a sensitivity of <0.056 ng/ml. Detection is colorimetric, performed on a microplate reader, with an assay time of 4h 30m on a pre-coated microplate platform arranged as 12 x 8 well strips. Kit components are stored at +4°C or -20°C as instructed and comprise a pre-coated 96 well microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers.

Reported recovery averages are 91% for serum (range 80%-102%), 91% for EDTA plasma (range 81%-101%), and 84% for heparin plasma (range 80%-89%). Linearity is provided across dilution factors 1:2, 1:4, 1:8, and 1:16 for serum, EDTA plasma, and heparin plasma samples.