Human Mitogen Activated Protein Kinase 13 ELISA Kit

Antibodies.com supplies a sandwich enzyme-linked immunosorbent assay for the in vitro quantitative determination of human p38 delta/MAPK13 in tissue homogenates or other biological fluids. The assay uses an antibody specific for p38 delta/MAPK13 pre-coated onto a 96-well microplate arranged as 12 x 8 well strips. Standards and samples are added to the wells; captured analyte is then detected with a Biotinylated Anti-p38 delta/MAPK13 Antibody, followed by an HRP-Avidin conjugate and TMB substrate. The enzymatic reaction produces a yellow product whose optical density is read at 450 nm on a colorimetric microplate reader, with concentration calculated against a standard curve.

Reported performance figures from the manufacturer include a sensitivity of 0.061 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4h 30m. Mean recovery values were 91% for serum (range 83%–100%), 94% for EDTA plasma (range 90%–98%), and 95% for heparin plasma (range 89%–102%). Reactivity is specified for human samples. Kit components are stored at +4°C or –20°C as labelled, and the kit should not be used past its expiration date.