Human Mitoferrin ELISA Kit

From Antibodies.com, this sandwich ELISA is listed in the catalogue as the Human Mitoferrin ELISA Kit with the target identified as Mitoferrin and is reactive with human samples. The manufacturer's accompanying product literature describes a Human SLC25A37 ELISA Kit designed for the in vitro quantitative determination of SLC25A37 in tissue homogenates or other biological fluids, with SLC25A37 listed as the protein target. According to that literature, the assay uses a pre-coated microplate, a biotinylated detection antibody, HRP-Avidin conjugate and TMB substrate, with absorbance read at 450 nm in a microplate reader.

Detection is colorimetric and total assay time is 4h 30m. Reported sensitivity is 0.053 ng/ml and the assay range is 0.156-10 ng/ml. Sample recovery in serum (n=5) was 80%-102% with an average of 91%, in EDTA plasma (n=5) 81%-100% averaging 90%, and in heparin plasma (n=5) 80%-89% averaging 84%. Dilution linearity was reported at 1:2, 1:4, 1:8 and 1:16 across serum, EDTA plasma and heparin plasma. Kit components are stored at +4°C or -20°C as instructed.