Human Microtubule Associated Protein 1 Light Chain 3 Alpha ELISA Kit

The Antibodies.com sandwich ELISA kit enables in vitro quantitative determination of human MAP1LC3A (Microtubule Associated Protein 1 Light Chain 3 Alpha) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated microplate format and a biotinylated anti-MAP1LC3A detection antibody with HRP-avidin and TMB substrate; absorbance is read at 450 nm using a colorimetric microplate reader.

Sensitivity is reported at 0.063 ng/ml and the assay range spans 0.156–10 ng/ml, with a total run time of approximately 4 hours 30 minutes. Mean recovery values were 91% for serum (range 83–100%, n=5), 95% for EDTA plasma (range 91–99%, n=5), and 94% for heparin plasma (range 90–99%, n=5). Linearity was demonstrated across 1:2, 1:4, 1:8, and 1:16 dilutions in these matrices. Reagents are stored at +4°C or -20°C as indicated, and the product is intended for research use only.