Human Methionyl Aminopeptidase 2 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is intended for the in vitro quantitative determination of human Methionyl Aminopeptidase 2 (also referred to as Methionine Aminopeptidase 2/p67) in tissue homogenates or other biological fluids. The kit uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection read at 450 nm in a microplate reader.

The assay has a stated detection range of 0.156–10 ng/ml and a sensitivity of 0.058 ng/ml, with a total assay time of 4 hours 30 minutes. Standards and samples are captured by an immobilised antibody specific for the target, then detected sequentially with a Biotinylated Anti-Methionyl Aminopeptidase 2/p67 Antibody and an HRP-Avidin conjugate. The reaction is visualised with TMB substrate, producing a blue product that turns yellow upon addition of an acidic stop solution; the intensity of yellow is proportional to target concentration, which is calculated against a standard curve.

Kit components should be stored at +4 °C or −20 °C as instructed, and the kit should not be used past its expiration date. Reported recovery values are 80–102% (average 91%) in serum, 81–101% (average 91%) in EDTA plasma and 80–89% (average 84%) in heparin plasma, with dilution linearity demonstrated from 1:2 to 1:16 across these same sample matrices.