Human Metastasis Associated In Colon Cancer 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human MACC1 (Metastasis Associated In Colon Cancer 1) in serum, plasma, tissue homogenates, or other biological fluids. The assay employs a pre-coated microplate together with a biotinylated anti-MACC1 antibody and HRP-avidin detection, with signal developed using TMB substrate and read at 450 nm on a colorimetric microplate reader.

The kit has a stated sensitivity of <0.127 ng/ml and an assay range of 0.312–20 ng/ml. It is suitable for serum, EDTA plasma, and heparin plasma samples. Reported average recovery is 91% for serum (range 80%–102%), 90% for EDTA plasma (range 81%–100%), and 84% for heparin plasma (range 80%–89%), each measured across n=5 samples. The total assay time is 4 hours 30 minutes, and kit components should be stored at +4°C or -20°C as instructed.