Human Metallothionein 1 ELISA Kit

Manufactured by Antibodies.com, this Human Metallothionein 1 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of human Metallothionein in serum, plasma, tissue homogenates or other biological fluids. The assay employs a pre-coated microplate (12 x 8 well strips) and uses a biotinylated anti-Metallothionein antibody plus HRP-avidin conjugate for detection, with TMB substrate and an acidic stop solution producing a yellow product that is read at 450 nm on a colorimetric microplate reader.

The kit has a stated sensitivity of <6.2 pg/ml and an assay range of 15.6-1000 pg/ml, with a total assay time of 4h 30m. Standard curve-based quantitation is supported by reported recovery values for serum (n=5, range 80%-102%, average 91%), EDTA plasma (n=5, range 81%-100%, average 90%), and heparin plasma (n=5, range 80%-89%, average 84%), along with dilution linearity across 1:2, 1:4, 1:8, and 1:16 sample dilutions for serum (87-91%, 87-107%, 74-101%, 92-97%), EDTA plasma (90-105%, 84-101%, 90-101%, 79-108%), and heparin plasma (84-95%, 92-105%, 82-105%, 89-91%). Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.