Human Membrane Protein, Palmitoylated 2 ELISA Kit

From the Antibodies.com catalogue, this sandwich ELISA kit is designed for the in vitro quantitative determination of human MPP2 (also known as DLG2) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay employs a 96-well microplate pre-coated with antibody specific for MPP2/DLG2. Standards and samples are added to the wells, followed by Biotinylated Anti-MPP2/DLG2 Antibody, HRP-Avidin conjugate and TMB substrate, producing a yellow colour whose density is read at 450 nm and is proportional to the amount of MPP2/DLG2 captured in each well.

The kit has a stated product range of 0.625-40 ng/ml and a sensitivity of 0.16 ng/ml, with colourmetric detection and a total assay time of 4h 30m. Manufacturer-reported recovery data include ranges of 80% - 102% (average 91%) for serum, 81% - 100% (average 90%) for EDTA plasma and 80% - 89% (average 84%) for heparin plasma, with further dilution-based recovery percentages provided across 1:2, 1:4, 1:8 and 1:16 sample dilutions for each matrix.

Components supplied include a pre-coated 96-well microplate arranged as 12 x 8 well strips, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, with storage temperatures specified for each item between +4°C and -20°C.