Human Matrix Metalloproteinase 28 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com for the in vitro quantitative determination of human Matrix Metalloproteinase 28 (MMP28) in tissue homogenates, cell lysates and other biological fluids. The assay reacts with human samples and is run on a pre-coated microtiter plate bearing a capture antibody specific for MMP28. Detection relies on a biotinylated anti-MMP28 antibody followed by an HRP-Avidin conjugate, producing a colorimetric signal that is read as absorbance at 450 nm in a microplate reader.

Manufacturer-stated performance figures include a sensitivity of 0.065 ng/ml and an assay range of 0.156–10 ng/ml, with a total run time of 4h 30m. The product is intended for research use only and is not for diagnostic or therapeutic applications. The supplied protocol advises bringing samples to room temperature before the assay, storing aliquots at low temperature for longer-term use and avoiding repeated freeze/thaw cycles.

Documented handling precautions include avoiding skin and eye contact with the acidic stop solution, limiting prolonged light exposure of the TMB substrate, and using separate reservoirs and pipette tips to prevent cross-contamination between standards, samples and reagents.