Human Matrix Metalloproteinase 25 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com designed for the in vitro quantitative determination of human MMP25 in serum, plasma, tissue homogenates and other biological fluids. The workflow uses a pre-coated 96-well microplate and a biotinylated anti-MMP25 detection antibody, with HRP-Avidin conjugate and TMB substrate producing a colour change read at 450 nm on a colorimetric microplate reader. Kit components are stored at +4°C or −20°C as instructed.

The quantitative assay range is 0.156–10 ng/ml with a reported sensitivity of <0.056 ng/ml, and the complete protocol takes 4 hours 30 minutes. The kit includes a pre-coated 96-well plate, lyophilised standard, detection reagents, wash and dilution buffers, TMB substrate, stop solution and plate sealers for quantitative MMP25 measurement in human-derived samples.