Human Major Histocompatibility Complex Class II DQ Beta 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human HLA DQB1 in tissue homogenates, cell lysates and other biological fluids, with reactivity confirmed for human samples. The target is Major Histocompatibility Complex Class II DQ Beta 1. Detection is performed using a pre-coated 96-well microplate (12 x 8 well strips) and a colorimetric microplate reader at 450 nm, with the protocol employing a biotinylated anti-HLA DQB1 detection antibody, an HRP-avidin conjugate, and TMB substrate.

The reported assay range is 0.312–20 ng/ml with a sensitivity of 0.116 ng/ml. Total assay time is 4 hours 30 minutes. Kit components should be stored at +4 °C or –20 °C as instructed, and the kit should not be used past its expiration date. Reported recovery values (n=5) include averages of 91% for serum (range 83–99%), 90% for EDTA plasma (range 80–100%) and 90% for heparin plasma (range 82–99%). Linearity was reported across dilutions of 1:2, 1:4, 1:8 and 1:16 in serum, EDTA plasma and heparin plasma.

The kit is described as suitable for cell lysates and tissue homogenates.