Human Latent Transforming Growth Factor Beta Binding Protein 2 ELISA Kit

From Antibodies.com, this Human LTBP2 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of human Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) in serum, plasma, tissue homogenates or other biological fluids. The assay is run on a pre-coated 96-well microplate (12 x 8 well strips) using a colourmetric detection method, with absorbance read at 450 nm in a microplate reader. HRP enzymatic activity is visualised with TMB substrate, producing a blue product that changes to yellow after the addition of acidic stop solution; the resulting absorbance is proportional to the amount of LTBP2 captured in each well.

Performance specifications include an assay range of 0.156–10 ng/ml and a sensitivity of <0.062 ng/ml. Validation across serum, EDTA plasma and Heparin plasma (n=5 each) yielded average recovery values of 91%, 90% and 84% respectively. Linearity testing at dilutions of 1:2, 1:4, 1:8 and 1:16 produced overall recovery ranges of 74–107% for serum, 79–108% for EDTA plasma and 82–105% for Heparin plasma. The complete assay can be performed in approximately 4h 30m.

Reagents supplied include a pre-coated microplate, lyophilised standard, biotinylated anti-LTBP2 detection antibody, HRP-avidin conjugate, wash buffer concentrate, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, with storage temperatures ranging between +4°C and -20°C as specified in the product documentation.