Human Laminin Alpha 4 ELISA Kit

Antibodies.com supplies a sandwich ELISA (sELISA) intended for the in vitro quantitative determination of human Laminin alpha 4 (LAMA4) in serum, plasma, or other biological fluids. The assay is run on a pre-coated 96-well microplate and read on a colorimetric microplate reader at 450 nm, with absorbance values used to calculate LAMA4 concentration against a standard curve.

The reported assay range is 0.625–40 ng/ml with a sensitivity of <0.238 ng/ml. Total assay time is 4 hours 30 minutes. Kit components are stored at +4°C or −20°C as instructed. The assay is suitable for serum and plasma samples.

In spike-and-recovery testing, average recoveries were 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5). Linearity was demonstrated across 1:2 to 1:16 dilutions for serum, EDTA plasma, and heparin plasma.