Human Kynurenine-3-Monooxygenase ELISA Kit

This sandwich ELISA kit from Antibodies.com enables the in vitro quantitative determination of human Kynurenine 3-Monooxygenase in tissue homogenates and other biological fluids. The assay uses a 96-well microplate pre-coated with a target-specific antibody. Captured analyte is detected with a biotinylated anti-Kynurenine 3-Monooxygenase antibody, an HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader and the concentration calculated from a standard curve.

Reported performance includes an assay range of 0.781–50 ng/ml and a sensitivity of <0.24 ng/ml, with a total assay time of 4h 30m. Components are stored at +4°C or -20°C and consist of a pre-coated 96-well microplate (12 x 8 well strips), lyophilized standard, Detection Solution A, Detection Solution B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Sample recovery ranges of 80-102% (serum, n=5), 81-100% (EDTA plasma, n=5), and 80-89% (heparin plasma, n=5) are reported, with dilution linearity demonstrated from 1:2 to 1:16 across matching matrices.