Human Kruppel Like Factor 15 ELISA Kit

Manufactured for Antibodies.com, this Human KLF15 ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the in vitro quantitative determination of human Kruppel-like factor 15 (KLF15) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a 96-well microtiter plate pre-coated with an antibody specific for KLF15, with detection via a biotinylated anti-KLF15 antibody, HRP-avidin conjugate, and TMB substrate, read colorimetrically at 450 nm.

The reported sensitivity is <0.068 ng/ml, with an assay range of 0.156–10 ng/ml. The total assay time is 4 hours 30 minutes. Recovery has been evaluated in serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, 1:8, and 1:16, with average recoveries of 91%, 96%, and 95% respectively. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.