Human Janus Kinase 1 ELISA Kit

Antibodies.com provides a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of human JAK1 in tissue homogenates, cell lysates, or other biological fluids. The assay employs a pre-coated 96-well microplate bearing an antibody specific for JAK1, with detection achieved using a biotinylated anti-JAK1 antibody and an HRP-avidin conjugate. The enzymatic reaction is visualised with TMB substrate, producing a blue product that turns yellow upon addition of acidic stop solution, and absorbance is read at 450 nm in a colorimetric microplate reader to calculate JAK1 concentration against a standard curve.

The kit reports a sensitivity of <0.051 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4 hours 30 minutes. It is validated for serum, EDTA plasma, and heparin plasma samples, showing average recovery values of 91%, 90%, and 84% respectively across these sample types, alongside dilution linearity data reported for 1:2, 1:4, 1:8, and 1:16 dilutions. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.