Human Interleukin-6 Soluble Receptor ELISA Kit

Human interleukin-6 (IL-6) signals through two distinct routes: classical signaling via the membrane-bound IL-6 receptor (IL-6R) and trans-signaling mediated by the soluble IL-6 receptor (sIL-6R). The sIL-6R isoform is produced by ADAM10/ADAM17-mediated ectodomain shedding of the membrane receptor and by alternative splicing of the IL6R transcript, generating a circulating factor that extends the cellular range of IL-6 responses. Quantifying sIL-6R supports studies of chronic inflammation, autoimmune disease activity, and tumor microenvironment biology.

This quantitative immunoassay from Antibodies.com is calibrated to detect human interleukin-6 soluble receptor in serum samples, plasma specimens, and conditioned cell culture supernatant. The kit is configured for 192 individual determinations and supplied in a pre-coated microwell plate strip format, along with calibrated protein standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, TMB substrate solution, and stop reagent.

Applied to cytokine pathway research, biomarker discovery in rheumatoid arthritis and inflammatory bowel disease, IL-6 trans-signaling mechanism studies, and pharmacodynamic monitoring of therapeutics that target the IL-6 axis. The assay is for in vitro research use only.