Human Interleukin 4 Induced Protein 1 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human IL-4I1/LAO (Interleukin 4 Induced Protein 1) in serum, plasma, tissue homogenates or other biological fluids. The format uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a biotinylated anti-IL-4I1/LAO detection antibody and an HRP-avidin conjugate. TMB substrate is used to visualize the reaction, producing a yellow product whose absorbance is read at 450 nm on a colorimetric microplate reader.

The assay reports a sensitivity of <0.052 ng/ml, a standard range of 0.156–10 ng/ml, and a total assay time of 4h 30m. Average recovery ranges from 84% to 91% across matrices: serum 91% (range 80–102%), EDTA plasma 90% (range 81–100%), and heparin plasma 84% (range 80–89%). Dilution linearity has been demonstrated at 1:2, 1:4, 1:8, and 1:16, with measured recoveries of 74–108% across the same sample types.

Included components are the pre-coated microplate, lyophilized standard, detection solution A, detection solution B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Storage instructions call for components to be kept at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.