Human Interleukin 28A ELISA Kit

Manufactured by Antibodies.com, this human Interleukin 28A (IL-28A) ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the in vitro quantitative determination of human IL-28A in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The workflow uses a pre-coated 96-well microplate in a 12 x 8 well strip format: standards and samples are added, followed by biotinylated anti-IL-28A antibody, HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. The reported sensitivity is <6.4 pg/ml and the product range is 15.6–1000 pg/ml. Total assay time is approximately 4 hours 30 minutes.

Sample recovery figures are reported for serum, EDTA plasma and heparin plasma, with dilution linearity data provided at 1:2, 1:4, 1:8 and 1:16 dilutions. Kit components include pre-coated 96-well strips, lyophilized standard, Detection Solution A and B, Wash Buffer (30X concentrate), Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, with the specified storage conditions listed in the product documentation.