Human Interleukin 17C ELISA Kit

Designed for the in vitro quantitative determination of human IL-17C, this sandwich ELISA from Antibodies.com is suitable for serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The kit provides quantitative measurement across a product range of 15.6-1000 pg/ml, with a sensitivity of 6.1 pg/ml, and is intended for use with a colorimetric microplate reader.

The assay uses a pre-coated microplate platform arranged as 12 x 8 well strips, with an antibody specific for IL-17C immobilised on the plate. Standards and test samples are added, followed by a Biotinylated Anti-IL-17C Antibody and an HRP-Avidin conjugate. TMB substrate is then used to visualise the HRP enzymatic reaction, with absorbance read at 450nm and concentration calculated against a standard curve. Total assay time is 4h 30m, and kit components should be stored at +4°C or -20°C as instructed, with the kit not to be used past its expiration date.

Recovery and dilution linearity were evaluated across serum, EDTA plasma, and heparin plasma. In spike-and-recovery testing (n=5), average recovery was 91% for serum (range 83-100%), 95% for EDTA plasma (range 91-100%), and 96% for heparin plasma (range 91-101%). Dilution linearity tested at 1:2, 1:4, 1:8, and 1:16 showed ranges of 78-110%, 84-96%, 90-101%, and 77-107% for serum; 93-96%, 89-109%, 72-106%, and 90-95% for EDTA plasma; and 84-104%, 82-101%, 95-101%, and 84-110% for heparin plasma.